Clear cell hepatocellular carcinoma: Gd-EOB-DTPA-enhanced MR imaging features and prognosis.

PURPOSE: To investigate imaging findings on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid-enhanced magnetic resonance imaging (Gd-EOB-DTPA-enhanced MRI) and prognosis of clear cell hepatocellular carcinoma (CCHCC) comparing with non-otherwise specified hepatocellular carcinoma (NOS-HCC). METHODS: The clinical, pathological and MR imaging features of 42 patients with CCHCC and 84 age-matched patients with NOS-HCC were retrospectively analyzed from January 2015 to October 2021. Univariate and multivariate logistic regression and Cox regression analyses were performed to identify independent diagnostic and prognostic factors for CCHCC. Disease-free survival (DFS) and overall survival (OS) were determined by Kaplan-Meier analysis. RESULTS: CCHCC showed fat content more frequently (P < 0.001) and relatively higher Edmondson tumor grade (P = 0.001) compared with NOS-HCC. The lesion-to-muscle ratio (LMR) and lesion-to-liver ratio (LLR) of CCHCC on pre-enhancement T1-weighted imaging (pre-T1WI) (P = 0.001, P = 0.003) and hepatobiliary phase (HBP) (P = 0.007, P = 0.048) were significantly higher than those of NOS-HCC. The area under the curve (AUC) for fat content, LLR on pre-T1WI and their combination with better diagnostic performance in predicting CCHCC were 0.678, 0.666, and 0.750, respectively. There was no statistically significant difference in clinical outcomes between CCHCC and NOS-HCC. Multivariate Cox analysis confirmed that tumor size > 2 cm and enhancing capsule were independent prognostic factors for DFS and OS among CCHCC patients. CONCLUSION: Fat content and adjusted lesion signal intensity on pre-T1WI and HBP could be used to differentiate CCHCC from NOS-HCC. CCHCC had similar prognosis with NOS-HCC.

An effective preserving strategy for strawberries by constructing pectin/starch coatings reinforced with functionalized eggshell fillers.

Food waste occurs frequently worldwide, though hunger and malnutrition issues have received global attention. Short-term spoilage of perishable foods causes a significant proportion of food waste. Developing simple, green, and low-cost strategies to preserve the freshness of perishable foods is important to address this issue and improving food safety. By using strawberries as the model perishable fruit, this study reported a pectin/carboxy methyl starch sodium (PC) based coating using epigallocatechin gallate-loaded eggshell powder (ES@EGCG) as the functional fillers. In comparison to PC coating, the PC-ES@EGCG coating displayed much-enhanced performance, such as enhanced mechanical (2 folds) and barrier (water vapor & oxygen) properties. This composite coating reduced the weight loss of strawberries from over 60% to around 30% after 7-day storage. Coated strawberries exhibit better freshness retention, which achieves the purpose of preserving strawberries during storage. This study provided a cost-effective and eco-friendly coating strategy for reducing food waste.

ctDNA transiting into urine is ultrashort and facilitates noninvasive liquid biopsy of HPV+ oropharyngeal cancer.

BACKGROUNDTransrenal cell-free tumor DNA (TR-ctDNA), which transits from the bloodstream into urine, has the potential to enable noninvasive cancer detection for a wide variety of nonurologic cancer types.MethodsUsing whole-genome sequencing, we discovered that urine TR-ctDNA fragments across multiple cancer types are predominantly ultrashort (<50 bp) and, therefore, likely to be missed by conventional ctDNA assays. We developed an ultrashort droplet digital PCR assay to detect TR-ctDNA originating from HPV-associated oropharyngeal squamous cell carcinoma (HPV+ OPSCC) and confirmed that assaying ultrashort DNA is critical for sensitive cancer detection from urine samples.ResultsTR-ctDNA was concordant with plasma ctDNA for cancer detection in patients with HPV+ OPSCC. As proof of concept for using urine TR-ctDNA for posttreatment surveillance, in a small longitudinal case series, TR-ctDNA showed promise for noninvasive detection of recurrence of HPV+ OPSCC.ConclusionOur data indicate that focusing on ultrashort fragments of TR-ctDNA will be important for realizing the full potential of urine-based cancer diagnostics. This has implications for urine-based detection of a wide variety of cancer types and for facilitating access to care through at-home specimen collections.FundingNIH grants R33 CA229023, R21 CA225493; NIH/National Cancer Institute grants U01 CA183848, R01 CA184153, and P30CA046592; American Cancer Society RSG-18-062-01-TBG; American Cancer Society Mission Boost grant MBGI-22-056-01-MBG; and the A. Alfred Taubman Medical Research Institute.

Safety risk assessment of subway shield construction under-crossing a river using CFA and FER.

Numerous subway projects are planned by China's city governments, and more subways can hardly avoid under-crossing rivers. While often being located in complex natural and social environments, subway shield construction under-crossing a river (SSCUR) is more susceptible to safety accidents, causing substantial casualties, and monetary losses. Therefore, there is an urgent need to investigate safety risks during SSCUR. The paper identified the safety risks during SSCUR by using a literature review and experts' evaluation, proposed a new safety risk assessment model by integrating confirmatory factor analysis (CFA) and fuzzy evidence reasoning (FER), and then selected a project to validate the feasibility of the proposed model. Research results show that (a) a safety risk list of SSCUR was identified, including 5 first-level safety risks and 38 second-level safety risks; (b) the proposed safety risk assessment model can be used to assess the safety risk of SSCUR; (c) safety inspection, safety organization and duty, quicksand layer, and high-pressure phreatic water were the high-level risks, and the onsite total safety risk was at the medium level; (d) management-type safety risks, environment-type safety risks, and personnel-type safety risks have higher expected utility values, and manager-type safety risks were expected have higher risk-utility values when compared to worker-type safety risks. The research can enrich the theoretical knowledge of SSCUR safety risk assessment and provide references to safety managers for conducting scientific and effective safety management on the construction site when a subway crosses under a river.

Histone lactylation inhibits RARγ expression in macrophages to promote colorectal tumorigenesis through activation of TRAF6-IL-6-STAT3 signaling.

Macrophages are phenotypically and functionally diverse in the tumor microenvironment (TME). However, how to remodel macrophages with a protumor phenotype and how to manipulate them for therapeutic purposes remain to be explored. Here, we show that in the TME, RARγ is downregulated in macrophages, and its expression correlates with poor prognosis in patients with colorectal cancer (CRC). In macrophages, RARγ interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6), which prevents TRAF6 oligomerization and autoubiquitination, leading to inhibition of nuclear factor κB signaling. However, tumor-derived lactate fuels H3K18 lactylation to prohibit RARγ gene transcription in macrophages, consequently enhancing interleukin-6 (IL-6) levels in the TME and endowing macrophages with tumor-promoting functions via activation of signal transducer and activator of transcription 3 (STAT3) signaling in CRC cells. We identified that nordihydroguaiaretic acid (NDGA) exerts effective antitumor action by directly binding to RARγ to inhibit TRAF6-IL-6-STAT3 signaling. This study unravels lactate-driven macrophage function remodeling by inhibition of RARγ expression and highlights NDGA as a candidate compound for treating CRC.

Predicting prognosis, immunotherapy and distinguishing cold and hot tumors in clear cell renal cell carcinoma based on anoikis-related lncRNAs.

Background: Clear cell renal cell carcinoma (ccRCC) is the most frequently occurring malignant tumor within the kidney cancer subtype. It has low sensitivity to traditional radiotherapy and chemotherapy, the optimal treatment for localized ccRCC has been surgical resection, but even with complete resection the tumor will be eventually developed into metastatic disease in up to 40% of localized ccRCC. For this reason, it is crucial to find early diagnostic and treatment markers for ccRCC. Methods: We obtained anoikis-related genes (ANRGs) integrated from Genecards and Harmonizome dataset. The anoikis-related risk model was constructed based on 12 anoikis-related lncRNAs (ARlncRNAs) and verified by principal component analysis (PCA), Receiver operating characteristic (ROC) curves, and T-distributed stochastic neighbor embedding (t-SNE), and the role of the risk score in ccRCC immune cell infiltration, immune checkpoint expression levels, and drug sensitivity was evaluated by various algorithms. Additionally, we divided patients based on ARlncRNAs into cold and hot tumor clusters using the ConsensusClusterPlus (CC) package. Results: The AUC of risk score was the highest among various factors, including age, gender, and stage, indicating that the model we built to predict survival was more accurate than the other clinical features. There was greater sensitivity to targeted drugs like Axitinib, Pazopanib, and Sunitinib in the high-risk group, as well as immunotherapy drugs. This shows that the risk-scoring model can accurately identify candidates for ccRCC immunotherapy and targeted therapy. Furthermore, our results suggest that cluster 1 is equivalent to hot tumors with enhanced sensitivity to immunotherapy drugs. Conclusion: Collectively, we developed a risk score model based on 12 prognostic lncRNAs, expected to become a new tool for evaluating the prognosis of patients with ccRCC, providing different immunotherapy strategies by screening for hot and cold tumors.

Wnt/ß-Catenin Signaling Pathway in the Development and Progression of Colorectal Cancer.

The Wnt/ß-catenin signaling pathway is a growth control pathway involved in various biological processes as well as the development and progression of cancer. Colorectal cancer (CRC) is one of the most common malignancies in the world. The hyperactivation of Wnt signaling is observed in almost all CRC and plays a crucial role in cancer-related processes such as cancer stem cell (CSC) propagation, angiogenesis, epithelial-mesenchymal transition (EMT), chemoresistance, and metastasis. This review will discuss how the Wnt/ß-catenin signaling pathway is involved in the carcinogenesis and progression of CRC and related therapeutic approaches.

Oxidation leaching of chromium from electroplating sludge: Ultrasonic enhancement and its mechanism.

The oxidation leaching of chromium from electroplating sludge was investigated, and ultrasonication was introduced for the enhancement of the leaching process. Two different types of Cr-bearing electroplating sludge were selected for the study, and the effects of the reagent dosage, temperature, and ultrasonic pulse ratio on the leaching efficiency were tested through oxidation leaching experiments. The experimental results show that hydrogen peroxide and sodium hypochlorite exhibit different leaching effects on different types of electroplating sludge. The control of reagent dosage is crucial for the oxidation leaching of Cr, while the effect of temperature turns out to be small. Hydrogen peroxide turns out to be a more effective oxidizer for chromium sludge, and the leaching efficiency of Cr could be promoted from 77.52% to 87.08% using ultrasonic enhancement under optimum conditions. Interestingly, sodium hypochlorite exhibited better leaching efficiency than hydrogen peroxide for the mixed sludge since the organic matter in the mixed sludge will lead to the rapid decomposition and consumption of hydrogen peroxide. The leaching efficiency of Cr from the mixed sludge could also be promoted from 56.82% to 67.10% using ultrasonic enhancement under optimum conditions. According to the scanning electron microscope imaging, ultrasonic enhancement can create voids and cracks on the surface of the sludge particles, hence promoting the contact between electroplating sludge and leaching agents, and promoting the oxidation leaching efficiency. In addition, ultrasound seems to be able to remove the coverings on the surface of the mixed sludge particles, which may facilitate the oxidation reaction.

Metagenomic and Microscopic Analysis of Magnetotactic Bacteria in Tangyin Hydrothermal Field of Okinawa Trough.

Magnetotactic bacteria (MTB) have been found in a wide variety of marine habitats, ranging from intertidal sediments to deep-sea seamounts. Deep-sea hydrothermal fields are rich in metal sulfides, which are suitable areas for the growth of MTB. However, MTB in hydrothermal fields have never been reported. Here, the presence of MTB in sediments from the Tangyin hydrothermal field was analyzed by 16S rRNA gene amplicon analysis, metagenomics, and transmission electron microscopy. Sequencing 16S rRNA gene yielded a total of 709 MTB sequences belonging to 20 OTUs, affiliated with Desulfobacterota, Alphaproteobacteria, and Nitrospirae. Three shapes of magnetofossil were identified by transmission electron microscopy: elongated-prismatic, bullet-shaped, and cuboctahedron. All of these structures were composed of Fe3O4. A total of 121 sequences were found to be homologous to the published MTB magnetosome-function-related genes, and relevant domains were identified. Further analysis revealed that diverse MTB are present in the Tangyin hydrothermal field, and that multicellular magnetotactic prokaryote (MMPs) might be the dominant MTB.

Magnetotactic bacteria and magnetofossils: ecology, evolution and environmental implications.

Magnetotactic bacteria (MTB) are a group of phylogenetically diverse and morphologically varied microorganisms with a magnetoresponsive capability called magnetotaxis or microbial magnetoreception. MTB are a distinctive constituent of the microbiome of aquatic ecosystems because they use Earth's magnetic field to align themselves in a north or south facing direction and efficiently navigate to their favored microenvironments. They have been identified worldwide from diverse aquatic and waterlogged microbiomes, including freshwater, saline, brackish and marine ecosystems, and some extreme environments. MTB play important roles in the biogeochemical cycling of iron, sulphur, phosphorus, carbon and nitrogen in nature and have been recognized from in vitro cultures to sequester heavy metals like selenium, cadmium, and tellurium, which makes them prospective candidate organisms for aquatic pollution bioremediation. The role of MTB in environmental systems is not limited to their lifespan; after death, fossil magnetosomal magnetic nanoparticles (known as magnetofossils) are a promising proxy for recording paleoenvironmental change and geomagnetic field history. Here, we summarize the ecology, evolution, and environmental function of MTB and the paleoenvironmental implications of magnetofossils in light of recent discoveries.

Oxytetracycline co-metabolism with denitrification/desulfurization in SRB mediated system.

Aquaculture wastewater contained a high remnant of oxytetracycline (OTC) and nitrate. In this study, OTC co-metabolized with denitrification/desulfurization was investigated in terms of kinetic analysis, pathway, microbial communities and produces analysis in sulfate-reducing bacteria (SRB) mediated system. Long-term acclimatization with sulfate (300 mg-S/L) could markedly accelerate the removed rate of OTC from 0.9 to 1.4 mg/g-SS/d, with the kinetic constants increasing from 0.2760 to 0.5232 d-1, mainly via enzymes including adenosine-5'-phos-phosulfate reductase and cytochrome P450, and non-enzymatic process related to intermediates (adenosine-5'-phos-phosulfate and S0). Furthermore, OTC was likely detoxified by SRB enriched sludge mainly via hydrolysis, dehydration, oxidation and reduction. The denitrification process would postpone the OTC degradation via outcompeting electron donors with the desulfurization process. Redundancy analysis suggested that sulfur-oxidizing bacteria (Acidithiobacillus, Ochrobactrum) were highly related to OTC degradation processes. This study provides deep insight and a new opportunity for the treatment of aquaculture wastewater containing OTC, sulfate and nitrate by SRB sludge.

Sulfidation forwarding high-strength Anammox process using nitrate as electron acceptor via thiosulfate-driven nitrate denitratation.

A single up-flow thiosulfate-driven nitrate denitratation coupled with the sulfurized Anammox (TDSA) with the core-shell structure (S0@ Anammox granules) provided a chemical/energy-saving way for the removal of high-content ammonium with nitrate as electron acceptor. Approximately 83.66% total nitrogen removal efficiency (TNRE) could be achieved by the sulfurized Anammox encrusted by S0/Sn2- at a high loading rate (2.6 kg-N/(m3·d)) via resisting high concentration of free ammonia (FA) (22.35 mg/L), mainly through S2O32-, S0/Sn2- -driven partial denitrification-Anammox (PDN-Anammox) process. Moreover, S0/Sn2--PDN-Anammox was largely restricted when intermittently aerated, but still resulting in 74.47% TNRE due to the partial nitrification-Anammox (PN-Anammox). The sequencing analysis revealed that Anammox bacterium (Candidatus_Kuenenia) and sulfur-oxidizing bacterium (Thiobacillus) coexisted, in which Anammox process occurred mainly via NO instead of NH2OH. This study provided a new perspective for high concentration nitrogen wastewater removal in engineering applications.

Electro-activating non-radical 1O2/H* via single atom manganese modified cathode: The indispensable role of metal active site Mn.

As an alternative to noble-metal Pt based catalysts, metal-based single atomic catalytic (SACs) exhibited excellent atom efficiency and catalytic activity via exposing abundant single atomic active centers. Here, we synthesized the monatomic Mn ligands anchored on porous N, P, S- co-doped carbon framework (Mn content over 4.5 wt%) (denoted as Mn-SAC@PZS). The single atomic Mn exhibited super mass activity (11.58 m2 g-1) and kinetic current (1.122×103 µA) with a much lower Tafel slope (4.25 mV dec-1) at 0.792 V (vs. SCE). XANES and EXAFS revealed that the mononuclear Mn were inclined to coordinate with N and S rather than P to form the R space of Mn, in which the first coordination shells backscattered with Mn-N and Mn-S. RRDE revealed that one-electron ORR pathway (72 ~ 100%) dominated at the potential of 0.5 ~ 0.7 V, oxygen molecule was absorbed/activated on site Mn* to form O* intermediate, then further activated to 1O2 via one-electron ORR pathway, while H* was electro activated by non-metallic active sites (i.e. pyri-N, sp-N, -PN and SO). In addition, the Mn-SAC@PZS was capable of highly selectively capturing and effectively degrading CIP in the presence of HA. Fast and complete removal of CIP was achieved within 30 min in the Mn-SAC@PZS-EFLP system, and the apparent rate constant (k) was up to 0.25 min-1. The energy consumption value was 0.453 kWh m-3, much lower than non-single atomic catalyst MnxOy@PZS (0.655 kWh m-3), which was comparable with the state-of-the-art advanced oxidation processes. These findings provided new insights into the maximum release of the atomic activity of the catalyst, and provides a possible way to selectively remove aromatics from multiple pollutants in complex water system.

No Light, No Germination: Excitation of the Rhodospirillum centenum Photosynthetic Apparatus Is Necessary and Sufficient for Cyst Germination.

Rhodospirillum centenum is a Gram-negative alphaproteobacterium that is capable of differentiating into dormant cysts that are metabolically inactive and desiccation resistant. Like spores synthesized by many Gram-positive species, dormant R. centenum cysts germinate in response to an environmental signal, indicating that conditions favor survival and proliferation. Factors that induce germination are called germinants and are often both niche and species specific. In this study, we have identified photosynthesis as a niche-specific germinant for R. centenum cyst germination. Specifically, excitation of wild-type cysts suspended in a nutrient-free buffer with far-red light at >750 nm results in rapid germination. This is in stark contrast to mutant strains deficient in photosynthesis that fail to germinate upon exposure to far-red light under all assayed conditions. We also show that photosynthesis-induced germination occurs in a carbon- and nitrogen-free buffer even in strains that are deficient in carbon or nitrogen fixation. These results demonstrate that photosynthesis not only is necessary for germination but is itself sufficient for the germination of R. centenum cysts.IMPORTANCE Environmental cues that signal Gram-positive spores to germinate (termed germinants) have been identified for several Bacillus and Clostridium species. These studies showed that germinants are niche and species specific. For example, Clostridium difficile spores sense bile salts as a germinant as their presence informs these cells of an intestinal environment. Bacillus fastidiosus spores use uric acid as a germinant that is present in soil and poultry litter as this species inhabits poultry litter. It is evident from these studies that dormant cells sample their environment to assess whether conditions are advantageous for the propagation and survival of vegetative cells. To date, a limited number of germinants have been defined for only a few Gram-positive spore-forming species. Beyond that group, there is scant information on what cues signal dormant cells to exit dormancy. In our study, we show that the versatile Gram-negative photosynthetic bacterium Rhodospirillum centenum uses light-driven photosynthesis, and not the availability of nutrients, to trigger the germination of dormant cysts. This use of light-driven photosynthesis as a germinant is surprising as this species is also capable of growing under dark conditions using exogenous carbon sources for energy. Consequently, photosynthetic growth appears to be the preferred growth mechanism by this species.

Gas-Sensing Performances of Metal Oxide Nanostructures for Detecting Dissolved Gases: A Mini Review.

Gas sensors have been wildly used in various fields related to people's lives. Gas sensor materials were the core factors that affected the performances of various gas sensors, and these have attracted much attention from scientific researchers due their high sensitivity, high selectivity, adjustable reliability, low cost, and other advantages. The preparation of nanostructures with a highly specific surface area was a useful method to improve the gas-sensing performance of a metal oxide semiconductor. Meanwhile, lots of research has focused on preparing nanostructures with a highly specific surface area. This paper has explored some fabricated sensors with high sensitivity, good selectivity, and long-term stability, which has also made them promising candidates for toxic gas detection. Besides, this paper has reviewed the development status of metal oxides used as gas sensors.

Quantifying Induced Polarization of Conductive Inclusions in Porous Media and Implications for Geophysical Measurements.

Induced polarization (IP) mapping has gained increasing attention in the past decades, as electrical induced polarization has been shown to provide interesting signatures for detecting the presence of geological materials such as clay, ore, pyrite, and potentially, hydrocarbons. However, efforts to relate complex conductivities associated with IP to intrinsic physical properties of the corresponding materials have been largely empirical. Here we present a quantitative interpretation of induced polarization signatures from brine-filled rock formations with conductive inclusions and show that new opportunities in geophysical exploration and characterization could arise. Initially tested with model systems with solid conductive inclusions, this theory is then extended and experimentally tested with nanoporous conductors that are shown to have a distinctive spectral IP response. Several of the tests were conducted with nano-porous sulfides (pyrite) produced by sulfate-reducing bacteria grown in the lab in the presence of a hydrocarbon source, as well as with field samples from sapropel formations. Our discoveries and fundamental understanding of the electrode polarization mechanism with solid and porous conductive inclusions suggest a rigorous new approach in geophysical exploration for mineral deposits. Moreover, we show how induced polarization of biologically generated mineral deposits can yield a new paradigm for basin scale hydrocarbon exploration.

Suppressing lithium dendrite formation by slowing its desolvation kinetics.

Slowing the dendrite formation process is one way to alleviate the fast capacity fade and safety issues in lithium metal battery systems. We used tetraethylene glycol dimethyl ether (TEGDME) as a complementary solvent to increase the desolvation activation energy of Li+, reduce the speed of lithium electrodeposition kinetics, and suppress dendrite formation. Density functional theory calculations combined with Raman spectroscopy indicate that a stronger coordination interaction is obtained between Li+ and TEGDME than between Li+ and 1,2-dimethoxyethane (DME) or 1,3-dioxolane (DOL). Such a strong coordination leads to a slower electrochemical reaction rate. As a result, uniform lithium electrodeposition morphology and good cycling stability of a Li|Li symmetric cell for more than 500 hours were achieved. Our approach suggests a way in which dendrite formation can be controlled by the electrochemical reaction itself.

Non-mono-exponential diffusion models for assessing early response of liver metastases to chemotherapy in colorectal Cancer.

BACKGROUND: Preoperative chemotherapy is becoming standard therapy for liver metastasis from colorectal cancer, so early assessment of treatment response is crucial to make a reasonable therapeutic regimen and avoid overtreatment, especially for patients with severe side effects. The role of three non-mono-exponential diffusion models, such as the kurtosis model, the stretched exponential model and the statistical model, were explored in this study to early assess the response to chemotherapy in patients with liver metastasis from colorectal cancer. METHODS: Thirty-three patients diagnosed as colorectal liver metastasis were evaluated in this study. Diffusion-weighted images with b values (0, 200, 500, 1000, 1500, 2000 s/mm2) were acquired at 3.0 T. The parameters (ADCk, K, DDC,α, Ds and σ) were derived from three non-mono-exponential models (the kurtosis, stretched exponential and statistical models) as well as their corresponding percentage changes before and after chemotherapy. The difference in above parameters between the response and non-response groups were analyzed with independent-samples T-test (normality) and Mann-Whitney U-test (non-normality). Meanwhile, receiver operating characteristic curve (ROC) analyses were performed to assess the response to chemotherapy. RESULTS: Significantly lower values of K (the kurtosis coefficient derived from the kurtosis model) and σ (the width of diffusion coefficient distribution in the statistical model) (P < 0.05) were observed in the respond group before treatment, as well as higher ΔK and Δσ values (P < 0.05) after the first cycle of chemotherapy were also found compared with the non-respond group. ROC analyses showed the K value acquired before treatment had the highest diagnostic performance (0.746) in distinguishing responders from non-responders. Furthermore, the high sensitivity (100%) and accuracy (76.3%) from the K value before treatment was found in assessing the response of colorectal liver metastasis to chemotherapy. CONCLUSIONS: The non-mono-exponential diffusion models may be able to predict early response to chemotherapy in patients with colorectal liver metastasis.